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pEX-N-GST載體質粒基本信息
pEX-N-GST質粒圖譜載體圖譜和pEX-N-GST載體序列質粒序列多克隆位點信息
pEX-N-GST質粒載體簡介
pEX-N-GST質粒序列載體序列
LOCUS pEX-N-GST 5200 bp DNA circular SYN 19-FEB-2014
DEFINITION PrecisionShuttle(TM) bacterial vector for inducible expression of a
protein with a cleavable N-terminal GST tag.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5200)
AUTHORS OriGene
TITLE Direct Submission
JOURNAL Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
//gt12365.cn
COMMENT Inserts are typically cloned between the SgfI (AsiSI) and MluI
sites.
COMMENT VNTDATE|690076800|
COMMENT VNTDBDATE|690076800|
COMMENT VNTNAME|pEX-N-GST|
COMMENT VNTAUTHORNAME|OriGene|
FEATURES Location/Qualifiers
promoter 218..236
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 237..261
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 281..286
/label=RBS
/note="ribosome binding site"
CDS 295..948
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
CDS 970..990
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV Site
misc_feature 991..1077
/label=MCS
/note="multiple cloning site"
misc_feature 1044..1046
/label=stop codon
terminator 1116..1162
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 1408..1485
/gene="lacI"
/label=lacI promoter
CDS 1486..2568
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
rep_origin complement(3019..3607)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3778..4638)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(4639..4743)
/gene="bla"
/label=AmpR promoter
rep_origin join(4770..5200,1..25)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
181 gtgccgaatt ctgctggact ttccaaaatg tcgagcttaa tacgactcac tataggggaa
241 ttgtgagcgg ataacaattc cccttttgtt taactttaag aaggagaggc caccatgtcc
301 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa
361 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga
421 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat
481 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg
541 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt
601 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt
661 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat
721 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt
781 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt
841 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct
901 ttgcagggct ggcaagccac gtttggtggt ggcgaccatc ctccaaaatc ggatatcatc
961 ccaacgaccg aaaacctgta ttttcagggc gcgatcgccg gcgcgccaga tctcaagctt
1021 aactagctag cggaccgacg cgttaaacgc ggccgctcga ggtttaaacg gccggccaca
1081 tgactgactg agccacgata accgctgagc aataactagc ataacccctt ggggcctcta
1141 aacgggtctt gaggggtttt ttcccaccag ccttgtccta atccgcggcg gttcctggcc
1201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
1261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
1321 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
1381 tgcggtattt cacaccgcat aaattccgac accatcgaat ggcgcaaaac ctttcgcggt
1441 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg
1501 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
1561 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
1621 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
1681 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
1741 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
1801 gcctgtaaag cggcggtgca caatcttctc gcgcaacgag tcagtgggct gatcattaac
1861 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
1921 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
1981 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
2041 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
2101 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
2161 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
2221 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
2281 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
2341 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
2401 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
2461 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
2521 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
2581 taatgtaagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
2641 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
2701 ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
2761 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
2821 gcaccgatgg atccgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
2881 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
2941 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
3001 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
3061 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
3121 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
3181 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
3241 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
3301 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
3361 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
3421 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
3481 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
3541 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
3601 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
3661 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
3721 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
3781 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
3841 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
3901 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
3961 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
4021 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
4081 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
4141 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
4201 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
4261 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
4321 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
4381 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
4441 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
4501 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
4561 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
4621 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
4681 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
4741 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
4801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
4861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
4921 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
4981 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
5041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
5101 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
5161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
//