LOCUS       HP4_M13        9447 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Helper plasmid for production of phagemid ssDNA, based on m13mp18.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz 
            H
  TITLE     Biotechnological mass production of DNA origami.
  JOURNAL   Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650.
  PUBMED    29219963
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-12-6- 6"; volume: "552"; issue: "7683"; pages:
            "84-87"
COMMENT     Sequence Label: HP4_M13
FEATURES             Location/Qualifiers
     source          1..9447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(83..112)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             323..1555
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGINLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVIWNETSRHRTLVAYLKHVELQHYIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             1567..1830
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             1832..1933
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             1930..2028
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     CDS             2025..2246
                     /codon_start=1
                     /gene="VIII"
                     /product="pVIII"
                     /label=M13 gene VIII
                     /note="major coat protein"
                     /translation="MKKSLVLKASVAVATLVPMLSFAAEGDDPAKAAFNSLQASATEYI
                     GYAWAMVVVIVGATIGIKLFKKFTSKAS"
     terminator      2250..2297
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             2302..3576
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKSHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDLFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENADENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             3579..3917
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             3919..4965
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             4943..6223
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      6266..6297
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(6396..7211)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7119..7138
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7203..7222
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(7569..8519)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      8567..8788
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(9280..9374)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ctttttcttt aaaaccgaaa agattacttc gcgtttgcca cctgacgtct aagaaaagga
       61 atattcagca atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag ccagtgagtt
      121 gattgctacg taattagtta gttagccctt agtgactcca gacgcgaatt atttttgatg
      181 gcgttcctat tggttaaaaa atgagctgat ttaacaaaaa tttaatgcga attttaacaa
      241 aatattaacg tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt
      301 ctgattatca accggggtac atatgattga catgctagtt ttacgattac cgttcatcga
      361 ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag atctctcaaa
      421 aatagctacc ctctccggca ttaatttatc agctagaacg gttgaatatc atattgatgg
      481 tgatttgact gtctccggcc tttctcaccc ttttgaatct ttacctacac attactcagg
      541 cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc
      601 ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt tagctttatg
      661 ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga
      721 tgttaatgct actactatta gtagaattga tgccaccttt tcagctcgcg ccccaaatga
      781 aaatatagct aaacaggtta ttgaccattt gcgaaatgta tctaatggtc aaactaaatc
      841 tactcgttcg cagaattggg aatcaactgt tatatggaat gaaacttcca gacaccgtac
      901 tttagttgca tatttaaaac atgttgagct acagcattat attcagcaat taagctctaa
      961 gccatccgca aaaatgacct cttatcaaaa ggagcaatta aaggtactct ctaatcctga
     1021 cctgttggag tttgcttccg gtctggttcg ctttgaagct cgaattaaaa cgcgatattt
     1081 gaagtctttc gggcttcctc ttaatctttt tgatgcaatc cgctttgctt ctgactataa
     1141 tagtcagggt aaagacctga tttttgattt atggtcattc tcgttttctg aactgtttaa
     1201 agcatttgag ggggattcaa tgaatattta tgacgattcc gcagtattgg acgctatcca
     1261 gtctaaacat tttactatta ccccctctgg caaaacttct tttgcaaaag cctctcgcta
     1321 ttttggtttt tatcgtcgtc tggtaaacga gggttatgat agtgttgctc ttactatgcc
     1381 tcgtaattcc ttttggcgtt atgtatctgc attagttgaa tgtggtattc ctaaatctca
     1441 actgatgaat ctttctacct gtaataatgt tgttccgtta gttcgtttta ttaacgtaga
     1501 tttttcttcc caacgtcctg actggtataa tgagccagtt cttaaaatcg cataaggtaa
     1561 ttcacaatga ttaaagttga aattaaacca tctcaagccc aatttactac tcgttctggt
     1621 gtttctcgtc agggcaagcc ttattcactg aatgagcagc tttgttacgt tgatttgggt
     1681 aatgaatatc cggttcttgt caagattact cttgatgaag gtcagccagc ctatgcgcct
     1741 ggtctgtaca ccgttcatct gtcctctttc aaagttggtc agttcggttc ccttatgatt
     1801 gaccgtctgc gcctcgttcc ggctaagtaa catggagcag gtcgcggatt tcgacacaat
     1861 ttatcaggcg atgatacaaa tctccgttgt actttgtttc gcgcttggta taatcgctgg
     1921 gggtcaaaga tgagtgtttt agtgtattct tttgcctctt tcgttttagg ttggtgcctt
     1981 cgtagtggca ttacgtattt tacccgttta atggaaactt cctcatgaaa aagtctttag
     2041 tcctcaaagc ctctgtagcc gttgctaccc tcgttccgat gctgtctttc gctgctgagg
     2101 gtgacgatcc cgcaaaagcg gcctttaact ccctgcaagc ctcagcgacc gaatatatcg
     2161 gttatgcgtg ggcgatggtt gttgtcattg tcggcgcaac tatcggtatc aagctgttta
     2221 agaaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc
     2281 ttttttttgg agattttcaa cgtgaaaaaa ttattattcg caattccttt agttgttcct
     2341 ttctattctc actccgctga aactgttgaa agttgtttag caaaatccca tacagaaaat
     2401 tcatttacta acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggc
     2461 tgtctgtgga atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt
     2521 acatgggttc ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc
     2581 ggttctgagg gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca
     2641 cctattccgg gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag
     2701 caaaaccccg ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg
     2761 tttcagaata ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt
     2821 actcaaggca ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc
     2881 atgtatgacg cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat
     2941 gaggatttat ttgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc
     3001 aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag
     3061 ggtggcggtt ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc
     3121 ggtgattttg attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc
     3181 gatgaaaacg cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac
     3241 ggtgctgcta tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct
     3301 actggtgatt ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca
     3361 cctttaatga ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc
     3421 ccttttgtct ttggcgctgg taaaccatat gaattttcta ttgattgtga caaaataaac
     3481 ttattccgtg gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct
     3541 acgtttgcta acatactgcg taataaggag tcttaatcat gccagttctt ttgggtattc
     3601 cgttattatt gcgtttcctc ggtttccttc tggtaacttt gttcggctat ctgcttactt
     3661 ttcttaaaaa gggcttcggt aagatagcta ttgctatttc attgtttctt gctcttatta
     3721 ttgggcttaa ctcaattctt gtgggttatc tctctgatat tagcgctcaa ttaccctctg
     3781 actttgttca gggtgttcag ttaattctcc cgtctaatgc gcttccctgt ttttatgtta
     3841 ttctctctgt aaaggctgct attttcattt ttgacgttaa acaaaaaatc gtttcttatt
     3901 tggattggga taaataatat ggctgtttat tttgtaactg gcaaattagg ctctggaaag
     3961 acgctcgtta gcgttggtaa gattcaggat aaaattgtag ctgggtgcaa aatagcaact
     4021 aatcttgatt taaggcttca aaacctcccg caagtcggga ggttcgctaa aacgcctcgc
     4081 gttcttagaa taccggataa gccttctata tctgatttgc ttgctattgg gcgcggtaat
     4141 gattcctacg atgaaaataa aaacggcttg cttgttctcg atgagtgcgg tacttggttt
     4201 aatacccgtt cttggaatga taaggaaaga cagccgatta ttgattggtt tctacatgct
     4261 cgtaaattag gatgggatat tatttttctt gttcaggact tatctattgt tgataaacag
     4321 gcgcgttctg cattagctga acatgttgtt tattgtcgtc gtctggacag aattacttta
     4381 ccttttgtcg gtactttata ttctcttatt actggctcga aaatgcctct gcctaaatta
     4441 catgttggcg ttgttaaata tggcgattct caattaagcc ctactgttga gcgttggctt
     4501 tatactggta agaatttgta taacgcatat gatactaaac aggctttttc tagtaattat
     4561 gattccggtg tttattctta tttaacgcct tatttatcac acggtcggta tttcaaacca
     4621 ttaaatttag gtcagaagat gaaattaact aaaatatatt tgaaaaagtt ttctcgcgtt
     4681 ctttgtcttg cgattggatt tgcatcagca tttacatata gttatataac ccaacctaag
     4741 ccggaggtta aaaaggtagt ctctcagacc tatgattttg ataaattcac tattgactct
     4801 tctcagcgtc ttaatctaag ctatcgctat gttttcaagg attctaaggg aaaattaatt
     4861 aatagcgacg atttacagaa gcaaggttat tcactcacat atattgattt atgtactgtt
     4921 tccattaaaa aaggtaattc aaatgaaatt gttaaatgta attaattttg ttttcttgat
     4981 gtttgtttca tcatcttctt ttgctcaggt aattgaaatg aataattcgc ctctgcgcga
     5041 ttttgtaact tggtattcaa agcaatcagg cgaatccgtt attgtttctc ccgatgtaaa
     5101 aggtactgtt actgtatatt catctgacgt taaacctgaa aatctacgca atttctttat
     5161 ttctgtttta cgtgcaaata attttgatat ggtaggttct aacccttcca ttattcagaa
     5221 gtataatcca aacaatcagg attatattga tgaattgcca tcatctgata atcaggaata
     5281 tgatgataat tccgctcctt ctggtggttt ctttgttccg caaaatgata atgttactca
     5341 aacttttaaa attaataacg ttcgggcaaa ggatttaata cgagttgtcg aattgtttgt
     5401 aaagtctaat acttctaaat cctcaaatgt attatctatt gacggctcta atctattagt
     5461 tgttagtgct cctaaagata ttttagataa ccttcctcaa ttcctttcaa ctgttgattt
     5521 gccaactgac cagatattga ttgagggttt gatatttgag gttcagcaag gtgatgcttt
     5581 agatttttca tttgctgctg gctctcagcg tggcactgtt gcaggcggtg ttaatactga
     5641 ccgcctcacc tctgttttat cttctgctgg tggttcgttc ggtattttta atggcgatgt
     5701 tttagggcta tcagttcgcg cattaaagac taatagccat tcaaaaatat tgtctgtgcc
     5761 acgtattctt acgctttcag gtcagaaggg ttctatctct gttggccaga atgtcccttt
     5821 tattactggt cgtgtgactg gtgaatctgc caatgtaaat aatccatttc agacgattga
     5881 gcgtcaaaat gtaggtattt ccatgagcgt ttttcctgtt gcaatggctg gcggtaatat
     5941 tgttctggat attaccagca aggccgatag tttgagttct tctactcagg caagtgatgt
     6001 tattactaat caaagaagta ttgctacaac ggttaatttg cgtgatggac agactctttt
     6061 actcggtggc ctcactgatt ataaaaacac ttctcaggat tctggcgtac cgttcctgtc
     6121 taaaatccct ttaatcggcc tcctgtttag ctcccgctct gattctaacg aggaaagcac
     6181 gttatacgtg ctcgtcaaag caaccatagt acgcgccctg tagcatgcag gagtcactaa
     6241 gggttagtta gttagattag cagaaagtca aaagcctccg accggaggct tttgactaaa
     6301 acttcccttg gggttatcat tggggctcac tcaaaggcgg taatcagata aaaaaaatcc
     6361 ttagctttcg ctaaggatga tttctgctag tattattaga aaaactcatc gagcatcaaa
     6421 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     6481 tgtaatgaag gagaaaactc accgaggcag ttccaaagaa tggcaaggtc ctggtaacgg
     6541 tctgcgattc cgacccgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     6601 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaagagc
     6661 ttgtgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     6721 ctcgcatcaa ccaaaccgtt attcatgcgt gattgcgcct gagcaagacg aaatacacga
     6781 tcgctgttaa aaggacaatt acaaacagga atcgaatgta accggcgcag gaacacggcc
     6841 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaaggctgtt
     6901 ttcccaggaa tcgcggtggt gagtaaccac gcatcatcag gagtacggat aaaatgcttg
     6961 atggtcggga gaggcataaa ctccgtcagc cagttgagac ggaccatctc atctgtaaca
     7021 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     7081 tacaagcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     7141 tataaatcag cgtccatgtt ggagtttaag cgcggacggg agcaagacgt ttcccgttga
     7201 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     7261 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     7321 tgttgaataa atcgaacttt tgctgagttg aaggatcagc tctagtagtt acattgtcga
     7381 tctgttcatg gtgaacagct ttgaatgcac caaaaactcg taaaagctct gatgtatcta
     7441 tcttttttac accgttttca tctgtgcata tggacagttt tccctttgat atgtaacggt
     7501 gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat acaagagcca
     7561 taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt tcgttgtttt
     7621 tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc actcaaaaat
     7681 tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag tgtttttctt
     7741 agtccgttat gtaggtagga atctgatgta atggttgttg gtattttgtc accattcatt
     7801 tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag ttcaacttgg
     7861 aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat attgctgtaa
     7921 gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt aaactcatgg
     7981 tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc tatatttgcc
     8041 ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct catagagtat
     8101 ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt taactggaaa
     8161 agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga gaacttggca
     8221 tagtttgtcc actggaaaat cccaaagcct ttaaccaaag gattcctgat ttccacagtt
     8281 ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc cctactgatg
     8341 ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt ccttgtaggg
     8401 ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt ttcatgctcc
     8461 gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa ttcagacata
     8521 catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc tagtcaatga
     8581 taattacatg tccttttcct ttgagttgtg ggtatctgta aattctgcta gacctttgct
     8641 ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt gtgtgttttt
     8701 ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa
     8761 gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa
     8821 ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa
     8881 gtagcaccct cgcaagctcg ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg
     8941 cacctgagtc gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg
     9001 aatgggggta aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata
     9061 atacaagaaa agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt
     9121 gctatctgac tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt
     9181 cggattatcc cgtgacaggt cattcagact ggctaatgca cccagtaagg cagcggtatc
     9241 atcaacaggc ttacccgtct tactgtccct agtgcttgga ttctcaccaa taaaaaacgc
     9301 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     9361 tatcaacagg agtccaagcg agctcgtaaa cttggtctga cagctctagc tccggcaaaa
     9421 aaacgggcaa ggtgtcacca ccctgcc
//