產品名稱: | saponlacus鹽鐵菌;ATCC培養基 2666 |
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英文名稱: | Haloferrarius saponlacus;ATCC medium 2666 |
培養基類型: | 加富培養基 |
級別: | for microbiology |
品牌: | ELITE-MEDIA(艾禮培養基) |
產品目錄號: | M2690 |
產品規格: | 250g、500g 添加劑請另購 |
產品外觀: | 乳白色至淺黃色粉末。 |
滅菌后顏色與澄清度: | 淺琥珀色,透明,無沉淀。 |
保存條件: | 密封,2-25°C保存。 |
注意事項: | 避免攝入、吸入、皮膚接觸。 |
相關產品: |
--Wolfe's Mineral Solution Trace Mineral Solution |
產品描述:
胰蛋白胨大豆肉湯(TSB)培養基是加富培養基,用于營養需求高的微生物(難養菌)的培養和回收,不需要加血清。胰蛋白胨大豆肉湯培養基適用范圍廣泛,可用于培養好氧菌、厭氧菌和真菌。例如:
Bacillus subtilis 枯草芽孢桿菌
Bacteroides vulgatus 普通擬桿菌
Candida albicans 白色念珠菌
Escherichia coli 大腸桿菌
Micrococcus luteus 藤黃微球菌
Staphylococcus aureus 金黃色葡萄球菌
Staphylococcus epidermidis 表皮葡萄球菌
Streptococcus pneumonia 肺炎鏈球菌
Streptococcus pyogenes 釀膿鏈球菌
用途:
作為致病菌的富集培養基。用于配制血液培養基和血瓊脂培養基。
用于無菌試驗,檢測真菌和好氧菌污染。
在醫學上,常用來培養從臨床樣品中分離的致病微生物。
用于藥敏試驗和紙片擴散法試驗。
配方與配制方法
成分 | g/L |
NaCl | 70.00 g |
NH4NO3 | 0.05 g |
Wolfe's Mineral Solution | 10.00 ml |
Trace Mineral Solution | 10.00 ml |
Na2SO4 | 13.50 g |
KH2PO4 | 3.00 g |
K2HPO | 3.00 g |
Methylene Blue | 1.00 mg |
Yeast extract | 1.00 g |
Na2BB 4O7 | 1.12 g |
Lactic acid | 5.00 g |
Fe-citrate | 20.00 mM |
Distilled water | 1.00 L |
用1000ml去離子水溶解96.7g培養基粉末。saponlacus鹽鐵菌滅菌條件是,121℃高壓蒸汽滅菌15min。
Adjust the pH to 8.5 with 1M NaOH. Boil and allow too cool under a steady stream of 80% N2 and 20% CO2. Add 10.0 g of Na2CO3 and 10.0 g of NaHCO3. Place the media into an anaerobic chamber and add 1.5 ml of a 10% cysteine hydrochloride solution. Note: the pH will increase to about 9.2 after adding the carbonate and the bicarbonate. Allow the media to remain overnight in the anaerobic chamber. Dispense 10 ml into Balch tubes and autoclave for 30 minutes at 121ºC. The methylene blue color reduction from blue to colorless is used as an indicator for anaerobic conditions. Limit exposure to light.
Trace Mineral Solution (g/l):
成分 | g/L |
CaSO4 | 0.500 g |
FeSO4 | 0.180 g |
SiO2 | 0.500 g |
MgCl2 | 0.850 g |
MnSO4 | 0.450 g |