LOCUS     pfwB       5904 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Buck CB, Thompson CD, Pang YY, Lowy DR, Schiller JT
  TITLE     Maturation of papillomavirus capsids.
  JOURNAL   J Virol. 2005 Mar;79(5):2839-46.
  PUBMED    15709003
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2005-03"; volume: "79(5)"; pages: "2839-46"
COMMENT     Sequence Label: pfwB
FEATURES             Location/Qualifiers
     source          1..5904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..73)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        58..387
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      238..373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        435..482
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     polyA_signal    962..1083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(973..992)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1027..1046
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1265..2443
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1496..2434
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2391..2411
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2554..2563
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2560..2562,2563..2565,2566..3279)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2560..2562,2563..2565,2566..3276)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2605..2626)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2866..2885)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3213..3234
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3309..3325
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3309..3325
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3363..3951
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3416..3436)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3834..3845)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3993..4565
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      4736..5324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5225..5244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5410..5457
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        5449..5467
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcaggg cctgaaataa cctctgaaag aggaacttgg ttaggtacct gtggaatgtg
       61 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
      181 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
      241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      301 atttatacag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      361 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
      421 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
      481 acaaggtgag gaactaaatc atgaagacct tcaacatctc tcagcaggat ctggagctgg
      541 tggaggtcgc cactgagaag atcaccatgc tctatgagga caacaagcac catgtcgggg
      601 cggccatcag gaccaagact ggggagatca tctctgctgt ccacattgag gcctacattg
      661 gcagggtcac tgtctgtgct gaagccattg ccattgggtc tgctgtgagc aacgggcaga
      721 aggactttga caccattgtg gctgtcaggc acccctactc tgatgaggtg gacagatcca
      781 tcagggtggt cagcccctgt ggcatgtgca gagagctcat ctctgactat gctcctgact
      841 gctttgtgct cattgagatg aatggcaagc tggtcaaaac caccattgag gaactcatcc
      901 ccctcaagta caccaggaac taaacctgaa ttcgctagag ggccgcttcg agcagacatg
      961 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1021 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1081 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1141 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gggctggcgt
     1201 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcggtggag aagagcatgc
     1261 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1321 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     1381 aagtgatgtc gtgtactggc tccgcccttt tcccgagggt gggggagaac cgtatataag
     1441 tgcagtagtc gctgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1501 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     1561 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     1621 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     1681 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     1741 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     1801 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     1861 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1921 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1981 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     2041 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     2101 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     2161 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     2221 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     2281 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2341 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2401 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tctagagatc
     2461 cctcgacctc gagatccatt gtgctggatc tgcgatctaa gtaagcttcg aattctgcag
     2521 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag
     2581 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     2641 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     2701 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     2761 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2821 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2881 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2941 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3001 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3061 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3121 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3181 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3241 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgctc caccgcggtg
     3301 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgatatc aagcttatcg
     3361 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     3961 gtcggcccac tgctccctaa acctgagcta gcattatccc taatacctgc caccccactc
     4021 ttaatcagtg gtggaagaac ggtctcagaa ctgtttgttt caattggcca tttaagttta
     4081 gtagtaaaag actggttaat gataacaatg catcgtaaaa ccttcagaag gaaaggagaa
     4141 tgttttgtgg accactttgg ttttcttttt tgcgtgtggc agttttaagt tattagtttt
     4201 taaaatcagt actttttaat ggaaacaact tgaccaaaaa tttgtcacag aattttgaga
     4261 cccattaaaa aagttaaatg agaaacctgt gtgttccttt ggtcaacacc gagacattta
     4321 ggtgaaagac atctaattct ggttttacga atctggaaac ttcttgaaaa tgtaattctt
     4381 gagttaacac ttctgggtgg agaatagggt tgttttcccc ccacataatt ggaaggggaa
     4441 ggaatatcat ttaaagctat gggagggttt ctttgattac aacactggag agaaatgcag
     4501 catgttgctg attgcctgtc actaaaacag gccaaaaact gagtccttgg gttgcataga
     4561 aagcttcatg ttgctaaacc aatgttaagt gaatctttgg aaacaaaatg tttccaaatt
     4621 actgggatgt gcatgttgaa acgtgggtta attaactagc catgaccaaa atcccttaac
     4681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttctta attaaatttt
     5401 tcaaaagtag ttgacaatta atcatcggca tagtatatcg gcatagtata atacgactca
     5461 ctataggagg gccatcatgg ccaagttgac cagtgctgtc ccagtgctca cagccaggga
     5521 tgtggctgga gctgttgagt tctggactga caggttgggg ttctccagag attttgtgga
     5581 ggatgacttt gcaggtgtgg tcagagatga tgtcaccctg ttcatctcag cagtccagga
     5641 ccaggtggtg cctgacaaca ccctggcttg ggtgtgggtg agaggactgg atgagctgta
     5701 tgctgagtgg agtgaggtgg tctccaccaa cttcagggat gccagtggcc ctgccatgac
     5761 agagattgga gagcagccct gggggagaga gtttgccctg agagacccag caggcaactg
     5821 tgtgcacttt gtggcagagg agcaggactg aggataagaa ttgtaacaaa aaaccccgcc
     5881 ccggcggggt tttttgttaa ttaa
//